Integrin nanoclusters can bridge thin matrix fibres to form cell–matrix adhesions
نویسندگان
چکیده
منابع مشابه
Integrin-Matrix Clusters Form Podosome-like Adhesions in the Absence of Traction Forces
Matrix-activated integrins can form different adhesion structures. We report that nontransformed fibroblasts develop podosome-like adhesions when spread on fluid Arg-Gly-Asp peptide (RGD)-lipid surfaces, whereas they habitually form focal adhesions on rigid RGD glass surfaces. Similar to classic macrophage podosomes, the podosome-like adhesions are protrusive and characterized by doughnut-shape...
متن کاملIntegrin adhesions
Cell migration requires the coordination of adhesion site assembly and turnover. Canonical models for nascent adhesion formation postulate that integrin binding to extracellular matrix (ECM) proteins results in the rapid recruitment of cytoskeletal proteins such as talin and paxillin to integrin cytoplasmic domains. It is thought that integrin-talin clusters recruit and activate tyrosine kinase...
متن کاملFocal adhesions require catalytic activity of Src family kinases to mediate integrin-matrix adhesion.
Members of the Src family of tyrosine kinases function to phosphorylate focal adhesion (FA) proteins. To explore the overlapping functions of Src kinases, we have targeted Csk, a negative regulator of the Src family, to FA structures. Expression of FA-targeted Csk (FA-Csk) effectively reduced the active form (nonphosphorylated at the C-terminal regulatory tyrosine) of Src members in the cell. W...
متن کاملIntegrin-Associated Complexes Form Hierarchically with Variable Stoichiometry in Nascent Adhesions
BACKGROUND A complex network of putative molecular interactions underlies the architecture and function of cell-matrix adhesions. Most of these interactions are implicated from coimmunoprecipitation studies using expressed components, but few have been demonstrated or characterized functionally in living cells. RESULTS We introduce fluorescence fluctuation methods to determine, at high spatia...
متن کاملFocal adhesions are sites of integrin extension
Integrins undergo global conformational changes that specify their activation state. Current models portray the inactive receptor in a bent conformation that upon activation converts to a fully extended form in which the integrin subunit leg regions are separated to enable ligand binding and subsequent signaling. To test the applicability of this model in adherent cells, we used a fluorescent r...
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ژورنال
عنوان ژورنال: Nature Materials
سال: 2019
ISSN: 1476-1122,1476-4660
DOI: 10.1038/s41563-019-0460-y